Electron microscopic mapping of Escherichia coli RNA polymerase binding sites on tetracycline resistant plasmid pNS1.

Electron microscopic mapping of wheat germ RNA polymerase II binding sites on cloned CaMV DNA.

The binding sites of wheat germ RNA polymerase II were mapped on the cloned CaMV genome by observation of enzyme-linear DNA complexes by electron microscopy. Twelve sites are observed. Three of them are relatively stable in the presence of heparin and are found at positions 8-9, 21-23, and 41-44 map units on the physical map of the genome. These positions correspond to AT-rich regions of the vi...

Binding sites on Escherichia coli ribonucleic acid polymerase.

Interconvertible forms of Escherichia coli RNA polymerase.

Escherichia coli RNA polymerase exists in two different forms, one less active than the other under standard assay conditions. Conversion of the active to the inactive form requires ATP-Mg(++). The reverse, inactive to active enzyme conversion, requires Mg(++). Covalently bound adenylic acid residue(s) are contained in the inactive form.

Cytoplasmic RNA Polymerase in Escherichia coli.

To obtain an estimate for the concentration of free functional RNA polymerase in the bacterial cytoplasm, the content of RNA polymerase beta and beta' subunits in DNA-free minicells from the minicell-producing Escherichia coli strain chi925 was determined. In bacteria grown in Luria-Bertani medium at 2.5 doublings/h, 1.0% of the total protein was RNA polymerase. The concentration of cytoplasmic...

Anatomy of Escherichia coli ribosome binding sites.

During translational initiation in prokaryotes, the 3' end of the 16S rRNA binds to a region just upstream of the initiation codon. The relationship between this Shine-Dalgarno (SD) region and the binding of ribosomes to translation start-points has been well studied, but a unified mathematical connection between the SD, the initiation codon and the spacing between them has been lacking. Using ...